LOCUS pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta... 13460 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Dox inducible expression of luciferase + crIFNg. Constitutive EF1a
driven rtTA and GFP .
ACCESSION .
VERSION .
KEYWORDS pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta...
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13460)
AUTHORS Kempton HR, Goudy LE, Love KS, Qi LS
TITLE Multiple Input Sensing and Signal Integration Using a Split Cas12a
System.
JOURNAL Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi:
10.1016/j.molcel.2020.01.016.
PUBMED 32027839
REFERENCE 2 (bases 1 to 13460)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13460
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 202..222
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
primer_bind complement(292..309)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(463..1051)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(543..562)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1222..2082)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1845..1864
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2083..2187)
/gene="bla"
/label=AmpR promoter
primer_bind 2255..2273
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2311..2333)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2433..2452
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2666..2685
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 2975..2993
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2975..2992
/label=SP6
/note="SP6 promoter, forward primer"
LTR 3018..3251
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind 3341..3357
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 3342..3358
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 3361..3949
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS 3467..3478
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 3876..3896
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4009..4755)
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
CDS complement(4768..4821)
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS complement(4831..5544)
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(4871..4891)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 5486..5514
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
intron 5569..6491
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind complement(5592..5612)
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 6686..6704
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 6722..6740
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 6758..6776
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 6794..6812
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 6830..6848
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 6866..6884
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 6902..6920
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 6965..6989
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 7066..8718
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(7132..7150)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
misc_feature 9106..9223
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(9508..9552)
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 9737..9970
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 10467..10592
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 10639..11272
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
polyA_signal 11440..11574
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(11999..12019)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 12149..12214
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
promoter complement(13348..217)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 13362..37
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(13416..13435)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
61 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
121 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
181 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgc attaatgaat
241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
1021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
1081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
1141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
1201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
1261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
1321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
1381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
1441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
1501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
1561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
1621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
1681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
1741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
1801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
1861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
1921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
1981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
2041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
2101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
2161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
2221 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
2281 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
2341 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
2401 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
2461 gcaccattcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
2521 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
2581 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
2641 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
2701 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcgatga
2761 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
2821 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
2881 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
2941 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaatacacg
3001 gaattaattc tagatgctgc tagagatttt ccacactgac taaaagggtc tgagggatct
3061 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
3121 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
3181 gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat
3241 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
3301 agtcattggt cttaaaggta ccgagctcga attaattccc tcgaggtcga cggtatcgat
3361 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
3421 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
3481 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
3541 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
3601 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
3661 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
3721 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
3781 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
3841 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
3901 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
3961 tgatatcaag cttgcatgcc tgcaggtcga ctctagagtc gcggccgctt agcctggcag
4021 catatcgaga tcgaagtcat cgagagcgtc agcagggagc atatccagat caaaatcatc
4081 cagggcatcg gcgggcagca tgtccaggtc gaagtcgtcc agggcgtcgg tgggtccgcc
4141 gctctcgcac ttcagctgct tctcgaggcc gcagatgatc agttccaggc cgaacaggaa
4201 ggcgggctcg gcgccctgtc tgtcgaacag ctcgatggcc tgcttcagca gggggggcat
4261 gctgtcggtg gtgggtgtct ctctttcctc tttggcgacc tggtgttcct gttcttccag
4321 cacgcagccc agggtgaagt ggcccacggc gctcagggcg tacagggcgt tttccaggct
4381 gaagccctgc tggcacagga aggccagctg gttttccagt gtctcgtact gcttctcggt
4441 gggtctggtg cccaggtgca ctttggcgcc gtcccggtgg ctcagcaggg cgcatctgta
4501 gctcttggcg ttgttccgca ggaagtcctg ccagctctcg ccttccagag ggcagctgtg
4561 ggtgtggtgc cggtccagca tctcgatggg cagggcgtcc agcagggccc gcttgttctt
4621 cacgtgccag tacagggtgg gctgttccac gcccagtttc tgggccagct tccgggtggt
4681 caggccctcg atgcccacgc cgttcagcag ttccagggcg ctgttgatca ctttgctctt
4741 gtccagccgg ctcatcgacc ctccgcctgg gccaggattc tcctcgacgt caccgcatgt
4801 tagcagactt cctctgccct cggaaccgcc tttgtagagc tcatccatgc catgtgtaat
4861 cccagcagca gttacaaact caagaaggac catgtggtca cgcttttcgt tgggatcttt
4921 cgaaaggaca gattgtgtcg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc
4981 aattggagta ttttgttgat aatggtctgc tagttgaacg gaaccatctt caacgttgtg
5041 gcgaattttg aagttagctt tgattccatt cttttgtttg tctgccgtga tgtatacatt
5101 gtgtgagtta aagttgtact cgagtttgtg tccaagaatg tttccatctt ctttaaaatc
5161 aatacccttt aactcgatac gattaacaag ggtatcacct tcaaacttga cttcagcacg
5221 cgtcttgtag gtcccgtcat ctttgaaaga tatagtgcgt tcctgtacat aaccttcggg
5281 catggcactc ttgaaaaagt catgccgttt catgtgatcc ggataacggg aaaagcattg
5341 aacaccatag gtcagagtag tgacaagtgt tggccacgga acaggtagtt ttccagtagt
5401 gcaaataaat ttaagggtga gttttccgtt tgtagcatca ccttcaccct ctccacggac
5461 agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga caactccagt
5521 gaaaagttct tctcctttgc tcatggtcgt acgcgttgtt cacgacacct gaaatggaag
5581 aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag
5641 gccaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca
5701 ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact
5761 ccaaaagctc cagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca
5821 tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg
5881 ctctcccgag cgccgcgtcc tccattttga gccccctgga gcagggccgg gaagcggcca
5941 tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat
6001 acacggcggc gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc
6061 gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg
6121 ggccccgtcg ccggccgcgg gcccaaaaac cgaaatacca gtgtgcagat cctggcccgc
6181 atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca cgtcatcaaa aattttaaat
6241 ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg
6301 gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg ctcctaaagc
6361 gcaaggcccg cccctggctc cagctcggga tcaagaatca cgtactggag ccaggtggaa
6421 gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac
6481 acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact
6541 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca
6601 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gcatatgctg
6661 atatctccac cccacagtgg tttactccct atcagtgata gagaacgtat gaagagttta
6721 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
6781 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
6841 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
6901 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
6961 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
7021 ataccaactt tccgtaccac ttcctaccct cgtaaagaat tcaccatgga agacgccaaa
7081 aacataaaga aaggcccggc gccattctat ccactagagg atggaaccgc tggagagcaa
7141 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
7201 catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa
7261 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
7321 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
7381 aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca gcctaccgta
7441 gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata
7501 atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
7561 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt accagagtcc
7621 tttgatcgtg acaaaacaat tgcactgata atgaactcct ctggatctac tgggttacct
7681 aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc cagagatcct
7741 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
7801 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
7861 tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat tcaaagtgcg
7921 ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga caaatacgat
7981 ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga agtcggggag
8041 gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct cactgagact
8101 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
8161 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
8221 cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt aaacaatccg
8281 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
8341 tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat taaatacaaa
8401 ggataccagg tggcccccgc tgaattggag tcgatattgt tacaacaccc caacatcttc
8461 gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
8521 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
8581 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
8641 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
8701 ggaaagtcca aattgtaagg atcctttctt tctttgaatt tctactaagt gtagatagat
8761 gagatggtga cagataggca atttctacta agtgtagata gatgagatgg tgacagatag
8821 gcaatttcta ctaagtgtag atcgagtttt ctttttgttt tgtacaagta agttacgcgt
8881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
8941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
9001 tcatgtctgg atcaactgga taactcaagc taaccgtcat actagtaatt cgatatcaag
9061 cttatcgatc caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
9121 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
9181 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
9241 catttggcga taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc
9301 tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt
9361 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
9421 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
9481 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
9541 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
9601 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
9661 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
9721 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
9781 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
9841 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
9901 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
9961 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
10021 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
10081 attgtccctc atatctcctc ctccaggtct gaagatcagc ggccggccgc ttgctgtgcg
10141 gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt
10201 atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt
10261 tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct
10321 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact
10381 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
10441 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
10501 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
10561 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
10621 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
10681 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
10741 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
10801 ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca ggatctgagg
10861 gctcgccact ccccagtccc gcccaggcca cgcctccctg gaaagtcccc agcggaaagt
10921 cccttgtagc aagctcgata tcagcagttc ttgaagtact ccggatgcag ctctcgggcc
10981 acgtgatgaa atgctaggcg gctgtcaaac ctccactcta acacttctct ctccgggtca
11041 tccatcccat gcaggctcac agggtgtaac aagctggtgt tctctccttt attggcctct
11101 tctaccttat ctggctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
11161 ctgacccctg gccctggtgt gtagttctgc taatcaggga agtagccttg tgtgtggtag
11221 atccacagat caaggatatc ttgtcttctt tgggagtgaa ttagcccttc caactactaa
11281 gtttgtagta catatttaac aaatacaatt tctttaaaat gaaaataatt cagaggaatc
11341 acaggtttag agtaaatgaa accacaggta attggcagtg gtaatagggt atggggtggg
11401 aagtttggga tgattttggt tagcttgagt tatccagttg atccagacat gataagatac
11461 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
11521 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
11581 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
11641 aagtaaaacc tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca
11701 tcaaatattc cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag
11761 catagttatt aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat
11821 gattataact gttatgccta cttataaagg ttacagaata tttttccata attttcttgt
11881 atagcagtgc agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac
11941 taaacacagc atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta
12001 cctttctctt cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac
12061 tagatggcat ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct
12121 cccattcatc agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt
12181 taacacatta tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta
12241 gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cggggcccac
12301 tcataaatcc agttgccgcc acggtagcca atcaccgtat cgtataaatc atcgtcggta
12361 cgttcggcat cgctcatcac aatacgtgcc tggacgtcga ggatttcgcg tgggtcaatg
12421 ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat ggttttacca
12481 tcaagggccg actgcacagg cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag
12541 gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg
12601 gatgcagcct ccagaatact tactggaaac tattgtaacc cgcctgaagt taaaaagaac
12661 aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg ggacgaatac
12721 gacgcccata tcccacggct gttcaatcca ggtatcttgc gggatatcaa caacatagtc
12781 atcaaccagc ggacgaccag ccggttttgc gaagatggtg acaaagtgcg cttttggata
12841 catttcacga atcgcaaccg cagtaccacc ggtatccacc aggtcatcaa taacgatgaa
12901 gccttcgcca tcgccttctg cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc
12961 gtagctggaa atacaaacgg tatcgacatg acgaataccc agttcacgcg ccagtaacgc
13021 acccggtaca gaccgccacg gcttacggca ataatgcctt tccattgttc agaaggcatc
13081 agtcggcttg cgagtttacg tgcatggatc tgcaacatgt cccaggtgac gatgtatttt
13141 tcgctcatgt gaagtgtccc agcctgttta tctacggctt aaaaagtgtt cgaggggaaa
13201 ataggttgcg cgagattata gagatctggc gcactaaaaa ccagtatttc acatgagtcc
13261 gcgtcttttt acgcactgcc tctccctgac gcgggataaa gtggtattct caaacatatc
13321 tcgcaagcct gtcttgtgtc caagcttttt gcaaaagcct aggcctccaa aaaagcctcc
13381 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
13441 aattagtcag ccatggggcg
//